Serological Response in RT-PCR Confirmed H1N1-2009 Influenza A by Hemagglutination Inhibition and Virus Neutralization Assays: An Observational Study
Identifieur interne : 000E27 ( Main/Exploration ); précédent : 000E26; suivant : 000E28Serological Response in RT-PCR Confirmed H1N1-2009 Influenza A by Hemagglutination Inhibition and Virus Neutralization Assays: An Observational Study
Auteurs : Mark I. Chen [Singapour] ; Ian G. Barr [Australie] ; Gerald C. H. Koh [Singapour] ; Vernon J. Lee [Singapour, Australie] ; Caroline P. S. Lee [Singapour] ; Robert Shaw [Australie] ; Cui Lin [Singapour] ; Jonathan Yap [Singapour] ; Alex R. Cook [Singapour] ; Boon Huan Tan [Singapour] ; Jin Phang Loh [Singapour] ; Timothy Barkham [Singapour] ; Vincent T. K. Chow [Singapour] ; Raymond T. P. Lin [Singapour] ; Yee-Sin Leo [Singapour]Source :
- PLoS ONE [ 1932-6203 ] ; 2010.
Abstract
We describe the serological response following H1N1-2009 influenza A infections confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR).
The study included patients admitted to hospital, subjects of a seroepidemiologic cohort study, and participants identified from outbreak studies in Singapore. Baseline (first available blood sample) and follow-up blood samples were analyzed for antibody titers to H1N1-2009 and recently circulating seasonal influenza A virus strains by hemagglutination inhibition (HI) and virus micro-neutralization (VM) assays. 267 samples from 118 cases of H1N1-2009 were analyzed. Geometric mean titers by HI peaked at 123 (95% confidence interval, CI 43-356) between days 30 to 39. The chance of observing seroconversion (four-fold or greater increase of antibodies) was maximized when restricting analysis to 45 participants with baseline sera collected within 5 days of onset and follow-up sera collected 15 or more days after onset; for these participants, 82% and 89% seroconverted to A/California/7/2009 H1N1 by HI and VM respectively. A four-fold or greater increase in cross-reactive antibody titers to seasonal A/Brisbane/59/2007 H1N1, A/Brisbane/10/2007 H3N2 and A/Wisconsin/15/2009 H3N2 occurred in 20%, 18% and 16% of participants respectively.
Appropriately timed paired serology detects 80–90% RT-PCR confirmed H1N1-2009; Antibodies from infection with H1N1-2009 cross-reacted with seasonal influenza viruses.
Url:
DOI: 10.1371/journal.pone.0012474
PubMed: 20814575
PubMed Central: 2930007
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000B14
- to stream Pmc, to step Curation: 000B14
- to stream Pmc, to step Checkpoint: 000895
- to stream Ncbi, to step Merge: 000564
- to stream Ncbi, to step Curation: 000564
- to stream Ncbi, to step Checkpoint: 000564
- to stream Main, to step Merge: 000E33
- to stream Main, to step Curation: 000E27
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Serological Response in RT-PCR Confirmed H1N1-2009 Influenza A by Hemagglutination Inhibition and Virus Neutralization Assays: An Observational Study</title><author><name sortKey="Chen, Mark I" sort="Chen, Mark I" uniqKey="Chen M" first="Mark I." last="Chen">Mark I. Chen</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Clinical Epidemiology, Tan Tock Seng Hospital, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Clinical Epidemiology, Tan Tock Seng Hospital, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2"><addr-line>Epidemiology and Public Health, National University of Singapore, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Epidemiology and Public Health, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Duke-NUS Graduate Medical School, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Duke-NUS Graduate Medical School, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Barr, Ian G" sort="Barr, Ian G" uniqKey="Barr I" first="Ian G." last="Barr">Ian G. Barr</name><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>World Health Organization Collaborating Centre for Reference and Research on Influenza, Melbourne, Victoria, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>World Health Organization Collaborating Centre for Reference and Research on Influenza, Melbourne, Victoria</wicri:regionArea><wicri:noRegion>Victoria</wicri:noRegion></affiliation></author><author><name sortKey="Koh, Gerald C H" sort="Koh, Gerald C H" uniqKey="Koh G" first="Gerald C. H." last="Koh">Gerald C. H. Koh</name><affiliation wicri:level="4"><nlm:aff id="aff2"><addr-line>Epidemiology and Public Health, National University of Singapore, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Epidemiology and Public Health, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation><affiliation wicri:level="4"><nlm:aff id="aff5"><addr-line>Centre for Health Services Research, National University of Singapore, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Centre for Health Services Research, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Lee, Vernon J" sort="Lee, Vernon J" uniqKey="Lee V" first="Vernon J." last="Lee">Vernon J. Lee</name><affiliation wicri:level="4"><nlm:aff id="aff2"><addr-line>Epidemiology and Public Health, National University of Singapore, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Epidemiology and Public Health, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation><affiliation wicri:level="4"><nlm:aff id="aff5"><addr-line>Centre for Health Services Research, National University of Singapore, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Centre for Health Services Research, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6"><addr-line>Biodefence Centre, Ministry of Defence, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Biodefence Centre, Ministry of Defence, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff7"><addr-line>Centre for Epidemiology and Population Health, Australian National University, Canberra, Australian Capital Territory, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Epidemiology and Population Health, Australian National University, Canberra, Australian Capital Territory</wicri:regionArea><wicri:noRegion>Australian Capital Territory</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Caroline P S" sort="Lee, Caroline P S" uniqKey="Lee C" first="Caroline P. S." last="Lee">Caroline P. S. Lee</name><affiliation wicri:level="1"><nlm:aff id="aff8"><addr-line>Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Shaw, Robert" sort="Shaw, Robert" uniqKey="Shaw R" first="Robert" last="Shaw">Robert Shaw</name><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>World Health Organization Collaborating Centre for Reference and Research on Influenza, Melbourne, Victoria, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>World Health Organization Collaborating Centre for Reference and Research on Influenza, Melbourne, Victoria</wicri:regionArea><wicri:noRegion>Victoria</wicri:noRegion></affiliation></author><author><name sortKey="Lin, Cui" sort="Lin, Cui" uniqKey="Lin C" first="Cui" last="Lin">Cui Lin</name><affiliation wicri:level="1"><nlm:aff id="aff9"><addr-line>National Public Health Laboratory, Ministry of Health, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>National Public Health Laboratory, Ministry of Health, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Yap, Jonathan" sort="Yap, Jonathan" uniqKey="Yap J" first="Jonathan" last="Yap">Jonathan Yap</name><affiliation wicri:level="1"><nlm:aff id="aff6"><addr-line>Biodefence Centre, Ministry of Defence, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Biodefence Centre, Ministry of Defence, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Cook, Alex R" sort="Cook, Alex R" uniqKey="Cook A" first="Alex R." last="Cook">Alex R. Cook</name><affiliation wicri:level="4"><nlm:aff id="aff10"><addr-line>Department of Statistics and Applied Probability, National University of Singapore, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Statistics and Applied Probability, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Tan, Boon Huan" sort="Tan, Boon Huan" uniqKey="Tan B" first="Boon Huan" last="Tan">Boon Huan Tan</name><affiliation wicri:level="1"><nlm:aff id="aff11"><addr-line>Detection and Diagnostics Laboratory, DSO National Laboratories, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Detection and Diagnostics Laboratory, DSO National Laboratories, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Loh, Jin Phang" sort="Loh, Jin Phang" uniqKey="Loh J" first="Jin Phang" last="Loh">Jin Phang Loh</name><affiliation wicri:level="1"><nlm:aff id="aff11"><addr-line>Detection and Diagnostics Laboratory, DSO National Laboratories, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Detection and Diagnostics Laboratory, DSO National Laboratories, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Barkham, Timothy" sort="Barkham, Timothy" uniqKey="Barkham T" first="Timothy" last="Barkham">Timothy Barkham</name><affiliation wicri:level="1"><nlm:aff id="aff12"><addr-line>Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Chow, Vincent T K" sort="Chow, Vincent T K" uniqKey="Chow V" first="Vincent T. K." last="Chow">Vincent T. K. Chow</name><affiliation wicri:level="4"><nlm:aff id="aff13"><addr-line>Department of Microbiology, National University of Singapore, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Microbiology, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Lin, Raymond T P" sort="Lin, Raymond T P" uniqKey="Lin R" first="Raymond T. P." last="Lin">Raymond T. P. Lin</name><affiliation wicri:level="1"><nlm:aff id="aff9"><addr-line>National Public Health Laboratory, Ministry of Health, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>National Public Health Laboratory, Ministry of Health, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Leo, Yee Sin" sort="Leo, Yee Sin" uniqKey="Leo Y" first="Yee-Sin" last="Leo">Yee-Sin Leo</name><affiliation wicri:level="1"><nlm:aff id="aff8"><addr-line>Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">20814575</idno><idno type="pmc">2930007</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2930007</idno><idno type="RBID">PMC:2930007</idno><idno type="doi">10.1371/journal.pone.0012474</idno><date when="2010">2010</date><idno type="wicri:Area/Pmc/Corpus">000B14</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000B14</idno><idno type="wicri:Area/Pmc/Curation">000B14</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000B14</idno><idno type="wicri:Area/Pmc/Checkpoint">000895</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000895</idno><idno type="wicri:Area/Ncbi/Merge">000564</idno><idno type="wicri:Area/Ncbi/Curation">000564</idno><idno type="wicri:Area/Ncbi/Checkpoint">000564</idno><idno type="wicri:Area/Main/Merge">000E33</idno><idno type="wicri:Area/Main/Curation">000E27</idno><idno type="wicri:Area/Main/Exploration">000E27</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Serological Response in RT-PCR Confirmed H1N1-2009 Influenza A by Hemagglutination Inhibition and Virus Neutralization Assays: An Observational Study</title><author><name sortKey="Chen, Mark I" sort="Chen, Mark I" uniqKey="Chen M" first="Mark I." last="Chen">Mark I. Chen</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Clinical Epidemiology, Tan Tock Seng Hospital, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Clinical Epidemiology, Tan Tock Seng Hospital, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="aff2"><addr-line>Epidemiology and Public Health, National University of Singapore, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Epidemiology and Public Health, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Duke-NUS Graduate Medical School, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Duke-NUS Graduate Medical School, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Barr, Ian G" sort="Barr, Ian G" uniqKey="Barr I" first="Ian G." last="Barr">Ian G. Barr</name><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>World Health Organization Collaborating Centre for Reference and Research on Influenza, Melbourne, Victoria, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>World Health Organization Collaborating Centre for Reference and Research on Influenza, Melbourne, Victoria</wicri:regionArea><wicri:noRegion>Victoria</wicri:noRegion></affiliation></author><author><name sortKey="Koh, Gerald C H" sort="Koh, Gerald C H" uniqKey="Koh G" first="Gerald C. H." last="Koh">Gerald C. H. Koh</name><affiliation wicri:level="4"><nlm:aff id="aff2"><addr-line>Epidemiology and Public Health, National University of Singapore, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Epidemiology and Public Health, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation><affiliation wicri:level="4"><nlm:aff id="aff5"><addr-line>Centre for Health Services Research, National University of Singapore, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Centre for Health Services Research, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Lee, Vernon J" sort="Lee, Vernon J" uniqKey="Lee V" first="Vernon J." last="Lee">Vernon J. Lee</name><affiliation wicri:level="4"><nlm:aff id="aff2"><addr-line>Epidemiology and Public Health, National University of Singapore, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Epidemiology and Public Health, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation><affiliation wicri:level="4"><nlm:aff id="aff5"><addr-line>Centre for Health Services Research, National University of Singapore, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Centre for Health Services Research, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6"><addr-line>Biodefence Centre, Ministry of Defence, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Biodefence Centre, Ministry of Defence, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff7"><addr-line>Centre for Epidemiology and Population Health, Australian National University, Canberra, Australian Capital Territory, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Epidemiology and Population Health, Australian National University, Canberra, Australian Capital Territory</wicri:regionArea><wicri:noRegion>Australian Capital Territory</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Caroline P S" sort="Lee, Caroline P S" uniqKey="Lee C" first="Caroline P. S." last="Lee">Caroline P. S. Lee</name><affiliation wicri:level="1"><nlm:aff id="aff8"><addr-line>Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Shaw, Robert" sort="Shaw, Robert" uniqKey="Shaw R" first="Robert" last="Shaw">Robert Shaw</name><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>World Health Organization Collaborating Centre for Reference and Research on Influenza, Melbourne, Victoria, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>World Health Organization Collaborating Centre for Reference and Research on Influenza, Melbourne, Victoria</wicri:regionArea><wicri:noRegion>Victoria</wicri:noRegion></affiliation></author><author><name sortKey="Lin, Cui" sort="Lin, Cui" uniqKey="Lin C" first="Cui" last="Lin">Cui Lin</name><affiliation wicri:level="1"><nlm:aff id="aff9"><addr-line>National Public Health Laboratory, Ministry of Health, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>National Public Health Laboratory, Ministry of Health, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Yap, Jonathan" sort="Yap, Jonathan" uniqKey="Yap J" first="Jonathan" last="Yap">Jonathan Yap</name><affiliation wicri:level="1"><nlm:aff id="aff6"><addr-line>Biodefence Centre, Ministry of Defence, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Biodefence Centre, Ministry of Defence, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Cook, Alex R" sort="Cook, Alex R" uniqKey="Cook A" first="Alex R." last="Cook">Alex R. Cook</name><affiliation wicri:level="4"><nlm:aff id="aff10"><addr-line>Department of Statistics and Applied Probability, National University of Singapore, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Statistics and Applied Probability, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Tan, Boon Huan" sort="Tan, Boon Huan" uniqKey="Tan B" first="Boon Huan" last="Tan">Boon Huan Tan</name><affiliation wicri:level="1"><nlm:aff id="aff11"><addr-line>Detection and Diagnostics Laboratory, DSO National Laboratories, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Detection and Diagnostics Laboratory, DSO National Laboratories, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Loh, Jin Phang" sort="Loh, Jin Phang" uniqKey="Loh J" first="Jin Phang" last="Loh">Jin Phang Loh</name><affiliation wicri:level="1"><nlm:aff id="aff11"><addr-line>Detection and Diagnostics Laboratory, DSO National Laboratories, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Detection and Diagnostics Laboratory, DSO National Laboratories, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Barkham, Timothy" sort="Barkham, Timothy" uniqKey="Barkham T" first="Timothy" last="Barkham">Timothy Barkham</name><affiliation wicri:level="1"><nlm:aff id="aff12"><addr-line>Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Chow, Vincent T K" sort="Chow, Vincent T K" uniqKey="Chow V" first="Vincent T. K." last="Chow">Vincent T. K. Chow</name><affiliation wicri:level="4"><nlm:aff id="aff13"><addr-line>Department of Microbiology, National University of Singapore, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Microbiology, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Lin, Raymond T P" sort="Lin, Raymond T P" uniqKey="Lin R" first="Raymond T. P." last="Lin">Raymond T. P. Lin</name><affiliation wicri:level="1"><nlm:aff id="aff9"><addr-line>National Public Health Laboratory, Ministry of Health, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>National Public Health Laboratory, Ministry of Health, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Leo, Yee Sin" sort="Leo, Yee Sin" uniqKey="Leo Y" first="Yee-Sin" last="Leo">Yee-Sin Leo</name><affiliation wicri:level="1"><nlm:aff id="aff8"><addr-line>Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore</wicri:regionArea><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>We describe the serological response following H1N1-2009 influenza A infections confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR).</p></sec><sec><title>Methodology and Principal Findings</title><p>The study included patients admitted to hospital, subjects of a seroepidemiologic cohort study, and participants identified from outbreak studies in Singapore. Baseline (first available blood sample) and follow-up blood samples were analyzed for antibody titers to H1N1-2009 and recently circulating seasonal influenza A virus strains by hemagglutination inhibition (HI) and virus micro-neutralization (VM) assays. 267 samples from 118 cases of H1N1-2009 were analyzed. Geometric mean titers by HI peaked at 123 (95% confidence interval, CI 43-356) between days 30 to 39. The chance of observing seroconversion (four-fold or greater increase of antibodies) was maximized when restricting analysis to 45 participants with baseline sera collected within 5 days of onset and follow-up sera collected 15 or more days after onset; for these participants, 82% and 89% seroconverted to A/California/7/2009 H1N1 by HI and VM respectively. A four-fold or greater increase in cross-reactive antibody titers to seasonal A/Brisbane/59/2007 H1N1, A/Brisbane/10/2007 H3N2 and A/Wisconsin/15/2009 H3N2 occurred in 20%, 18% and 16% of participants respectively.</p></sec><sec><title>Conclusions and Significance</title><p>Appropriately timed paired serology detects 80–90% RT-PCR confirmed H1N1-2009; Antibodies from infection with H1N1-2009 cross-reacted with seasonal influenza viruses.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Dawood, Fs" uniqKey="Dawood F">FS Dawood</name></author><author><name sortKey="Jain, S" uniqKey="Jain S">S Jain</name></author><author><name sortKey="Finelli, L" uniqKey="Finelli L">L Finelli</name></author><author><name sortKey="Shaw, Mw" uniqKey="Shaw M">MW Shaw</name></author><author><name sortKey="Lindstrom, S" uniqKey="Lindstrom S">S Lindstrom</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fraser, C" uniqKey="Fraser C">C Fraser</name></author><author><name sortKey="Donnelly, Ca" uniqKey="Donnelly C">CA Donnelly</name></author><author><name sortKey="Cauchemez, S" uniqKey="Cauchemez S">S Cauchemez</name></author><author><name sortKey="Hanage, Wp" uniqKey="Hanage W">WP Hanage</name></author><author><name sortKey="Van Kerkhove, Md" uniqKey="Van Kerkhove M">MD Van Kerkhove</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ong, Jbs" uniqKey="Ong J">JBS Ong</name></author><author><name sortKey="Chen, Mic" uniqKey="Chen M">MIC Chen</name></author><author><name sortKey="Cook, Ar" uniqKey="Cook A">AR Cook</name></author><author><name sortKey="Lee, Hc" uniqKey="Lee H">HC Lee</name></author><author><name sortKey="Lee, Vj" uniqKey="Lee V">VJ Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ross, T" uniqKey="Ross T">T Ross</name></author><author><name sortKey="Zimmer, S" uniqKey="Zimmer S">S Zimmer</name></author><author><name sortKey="Burke, D" uniqKey="Burke D">D Burke</name></author><author><name sortKey="Crevar, C" uniqKey="Crevar C">C Crevar</name></author><author><name sortKey="Carter, D" uniqKey="Carter D">D Carter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Flahault, A" uniqKey="Flahault A">A Flahault</name></author><author><name sortKey="De Lamballerie, X" uniqKey="De Lamballerie X">X de Lamballerie</name></author><author><name sortKey="Hanslik, T" uniqKey="Hanslik T">T Hanslik</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miller, E" uniqKey="Miller E">E Miller</name></author><author><name sortKey="Hoschler, K" uniqKey="Hoschler K">K Hoschler</name></author><author><name sortKey="Hardelid, P" uniqKey="Hardelid P">P Hardelid</name></author><author><name sortKey="Stanford, E" uniqKey="Stanford E">E Stanford</name></author><author><name sortKey="Andrews, N" uniqKey="Andrews N">N Andrews</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Mic" uniqKey="Chen M">MIC Chen</name></author><author><name sortKey="Lee, Vjm" uniqKey="Lee V">VJM Lee</name></author><author><name sortKey="Lim, W Y" uniqKey="Lim W">W-Y Lim</name></author><author><name sortKey="Barr, Ig" uniqKey="Barr I">IG Barr</name></author><author><name sortKey="Lin, Rtp" uniqKey="Lin R">RTP Lin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nolan, T" uniqKey="Nolan T">T Nolan</name></author><author><name sortKey="Mcvernon, J" uniqKey="Mcvernon J">J McVernon</name></author><author><name sortKey="Skeljo, M" uniqKey="Skeljo M">M Skeljo</name></author><author><name sortKey="Richmond, P" uniqKey="Richmond P">P Richmond</name></author><author><name sortKey="Wadia, U" uniqKey="Wadia U">U Wadia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Greenberg, Me" uniqKey="Greenberg M">ME Greenberg</name></author><author><name sortKey="Lai, Mh" uniqKey="Lai M">MH Lai</name></author><author><name sortKey="Hartel, Gf" uniqKey="Hartel G">GF Hartel</name></author><author><name sortKey="Wichems, Ch" uniqKey="Wichems C">CH Wichems</name></author><author><name sortKey="Gittleson, C" uniqKey="Gittleson C">C Gittleson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hancock, K" uniqKey="Hancock K">K Hancock</name></author><author><name sortKey="Veguilla, V" uniqKey="Veguilla V">V Veguilla</name></author><author><name sortKey="Lu, X" uniqKey="Lu X">X Lu</name></author><author><name sortKey="Zhong, W" uniqKey="Zhong W">W Zhong</name></author><author><name sortKey="Butler, En" uniqKey="Butler E">EN Butler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zambon, M" uniqKey="Zambon M">M Zambon</name></author><author><name sortKey="Hays, J" uniqKey="Hays J">J Hays</name></author><author><name sortKey="Webster, A" uniqKey="Webster A">A Webster</name></author><author><name sortKey="Newman, R" uniqKey="Newman R">R Newman</name></author><author><name sortKey="Keene, O" uniqKey="Keene O">O Keene</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Beyer, We" uniqKey="Beyer W">WE Beyer</name></author><author><name sortKey="Palache, Am" uniqKey="Palache A">AM Palache</name></author><author><name sortKey="Luchters, G" uniqKey="Luchters G">G Luchters</name></author><author><name sortKey="Nauta, J" uniqKey="Nauta J">J Nauta</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cowling, Bj" uniqKey="Cowling B">BJ Cowling</name></author><author><name sortKey="Chan, Kh" uniqKey="Chan K">KH Chan</name></author><author><name sortKey="Fang, Vj" uniqKey="Fang V">VJ Fang</name></author><author><name sortKey="Lau, Llh" uniqKey="Lau L">LLH Lau</name></author><author><name sortKey="So, Hc" uniqKey="So H">HC So</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tay, J" uniqKey="Tay J">J Tay</name></author><author><name sortKey="Ng, Yf" uniqKey="Ng Y">YF Ng</name></author><author><name sortKey="Cutter, Jl" uniqKey="Cutter J">JL Cutter</name></author><author><name sortKey="James, L" uniqKey="James L">L James</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Mi" uniqKey="Chen M">MI Chen</name></author><author><name sortKey="Lee, Vj" uniqKey="Lee V">VJ Lee</name></author><author><name sortKey="Barr, Ig" uniqKey="Barr I">IG Barr</name></author><author><name sortKey="Lin, C" uniqKey="Lin C">C Lin</name></author><author><name sortKey="Lim, Wy" uniqKey="Lim W">WY Lim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, Vj" uniqKey="Lee V">VJ Lee</name></author><author><name sortKey="Yap, J" uniqKey="Yap J">J Yap</name></author><author><name sortKey="Cook, Ar" uniqKey="Cook A">AR Cook</name></author><author><name sortKey="Chen, Mi" uniqKey="Chen M">MI Chen</name></author><author><name sortKey="Tay, Jk" uniqKey="Tay J">JK Tay</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Leo, Ys" uniqKey="Leo Y">YS Leo</name></author><author><name sortKey="Lye, Dc" uniqKey="Lye D">DC Lye</name></author><author><name sortKey="Barkham, T" uniqKey="Barkham T">T Barkham</name></author><author><name sortKey="Krishnan, P" uniqKey="Krishnan P">P Krishnan</name></author><author><name sortKey="Seow, E" uniqKey="Seow E">E Seow</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yin Murphy, M" uniqKey="Yin Murphy M">M Yin-Murphy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Epstein, Sl" uniqKey="Epstein S">SL Epstein</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Australie</li><li>Singapour</li></country><orgName><li>Université nationale de Singapour</li></orgName></list><tree><country name="Singapour"><noRegion><name sortKey="Chen, Mark I" sort="Chen, Mark I" uniqKey="Chen M" first="Mark I." last="Chen">Mark I. Chen</name></noRegion><name sortKey="Barkham, Timothy" sort="Barkham, Timothy" uniqKey="Barkham T" first="Timothy" last="Barkham">Timothy Barkham</name><name sortKey="Chen, Mark I" sort="Chen, Mark I" uniqKey="Chen M" first="Mark I." last="Chen">Mark I. Chen</name><name sortKey="Chen, Mark I" sort="Chen, Mark I" uniqKey="Chen M" first="Mark I." last="Chen">Mark I. Chen</name><name sortKey="Chow, Vincent T K" sort="Chow, Vincent T K" uniqKey="Chow V" first="Vincent T. K." last="Chow">Vincent T. K. Chow</name><name sortKey="Cook, Alex R" sort="Cook, Alex R" uniqKey="Cook A" first="Alex R." last="Cook">Alex R. Cook</name><name sortKey="Koh, Gerald C H" sort="Koh, Gerald C H" uniqKey="Koh G" first="Gerald C. H." last="Koh">Gerald C. H. Koh</name><name sortKey="Koh, Gerald C H" sort="Koh, Gerald C H" uniqKey="Koh G" first="Gerald C. H." last="Koh">Gerald C. H. Koh</name><name sortKey="Lee, Caroline P S" sort="Lee, Caroline P S" uniqKey="Lee C" first="Caroline P. S." last="Lee">Caroline P. S. Lee</name><name sortKey="Lee, Vernon J" sort="Lee, Vernon J" uniqKey="Lee V" first="Vernon J." last="Lee">Vernon J. Lee</name><name sortKey="Lee, Vernon J" sort="Lee, Vernon J" uniqKey="Lee V" first="Vernon J." last="Lee">Vernon J. Lee</name><name sortKey="Lee, Vernon J" sort="Lee, Vernon J" uniqKey="Lee V" first="Vernon J." last="Lee">Vernon J. Lee</name><name sortKey="Leo, Yee Sin" sort="Leo, Yee Sin" uniqKey="Leo Y" first="Yee-Sin" last="Leo">Yee-Sin Leo</name><name sortKey="Lin, Cui" sort="Lin, Cui" uniqKey="Lin C" first="Cui" last="Lin">Cui Lin</name><name sortKey="Lin, Raymond T P" sort="Lin, Raymond T P" uniqKey="Lin R" first="Raymond T. P." last="Lin">Raymond T. P. Lin</name><name sortKey="Loh, Jin Phang" sort="Loh, Jin Phang" uniqKey="Loh J" first="Jin Phang" last="Loh">Jin Phang Loh</name><name sortKey="Tan, Boon Huan" sort="Tan, Boon Huan" uniqKey="Tan B" first="Boon Huan" last="Tan">Boon Huan Tan</name><name sortKey="Yap, Jonathan" sort="Yap, Jonathan" uniqKey="Yap J" first="Jonathan" last="Yap">Jonathan Yap</name></country><country name="Australie"><noRegion><name sortKey="Barr, Ian G" sort="Barr, Ian G" uniqKey="Barr I" first="Ian G." last="Barr">Ian G. Barr</name></noRegion><name sortKey="Lee, Vernon J" sort="Lee, Vernon J" uniqKey="Lee V" first="Vernon J." last="Lee">Vernon J. Lee</name><name sortKey="Shaw, Robert" sort="Shaw, Robert" uniqKey="Shaw R" first="Robert" last="Shaw">Robert Shaw</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E27 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000E27 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:2930007
|texte= Serological Response in RT-PCR Confirmed H1N1-2009 Influenza A by Hemagglutination Inhibition and Virus Neutralization Assays: An Observational Study
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:20814575" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |